Generator

Part:BBa_K339005

Designed by: Emily Hicks   Group: iGEM10_Calgary   (2010-10-25)

mutant malE Protein Generator

Circuit to produce mutant maltose binding protein. malE31 expression is being driven by an arabinose inducible promoter and a strong Ribosomal binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1671
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1407
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1369
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None